Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
 
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve
Pubget Overpricing |  
lüll
Positive contribution of ERdj5/JPDI to endoplasmic reticulum protein quality control in the salivary gland Hosoda A; Tokuda M; Akai R; Kohno K; Iwawaki TBiochem J 2009[Sep]; 425 (1): 117-25In eukaryotic cells, most membrane and secretory proteins are modified post-translationally in the ER (endoplasmic reticulum) for correct folding and assembly. Disulfide-bond formation is one of the important modifications affecting folding and is catalysed by the PDI (protein disulfide isomerase) family proteins. ERdj5 [also known as JPDI (J-domain-containing PDI-like protein)] is a member of the PDI family proteins and has been reported to act as a reductase in ERAD (ER-associated degradation). However, the role of ERdj5 at the whole-body level remains unclear. Therefore in the present study we generated ERdj5-knockout mice the mouse gene of ERdj5 is known as Dnajc10 [DnaJ (Hsp40) homologue, subfamily C, member 10] and analysed them. Although ERdj5-knockout mice were viable and healthy, the ER stress response was activated in the salivary gland of the knockout mice more than that of control mice. Furthermore, in ERdj5-knockout cells, the expression of exogenous ERdj5 mitigated the ER stress caused by overproduction of alpha-amylase, which is one of the most abundant proteins in saliva and has five intramolecular disulfide bonds. This effect was dependent on the thioredoxin-like motifs of ERdj5. Thus we suggest that ERdj5 contributes to ER protein quality control in the salivary gland.|*Protein Processing, Post-Translational[MESH]|Animals[MESH]|Blotting, Western[MESH]|Cells, Cultured[MESH]|Disulfides/metabolism[MESH]|Embryo, Mammalian/cytology[MESH]|Endoplasmic Reticulum/*metabolism[MESH]|Female[MESH]|Fibroblasts/cytology/metabolism[MESH]|HSP40 Heat-Shock Proteins/genetics/*metabolism[MESH]|Male[MESH]|Mice[MESH]|Mice, Inbred C57BL[MESH]|Mice, Knockout[MESH]|Microscopy, Electron, Transmission[MESH]|Molecular Chaperones/genetics/*metabolism[MESH]|Protein Folding[MESH]|Proteins/chemistry/metabolism[MESH]|Salivary Glands/*metabolism/ultrastructure[MESH]|alpha-Amylases/metabolism[MESH] |
