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lüll Induction of cytokine gene expression in human thyroid epithelial cells irradiated with HZE particles (iron ions) Sanzari JK; Nuth M; Kennedy ARRadiat Res 2009[Oct]; 172 (4): 437-43Gene expression profiles were examined using cDNA microarray technology in human thyroid epithelial (Htori-3) cells exposed to a low, non-toxic dose (10 cGy) of radiation from HZE particles in the form of iron ions in the absence or presence of selenomethionine (SeM). A total of 215 genes were differentially regulated 2 h after exposure to a 10-cGy dose of iron-ion radiation. In the microarray analysis, SeM had profound effects on the radiation-induced expression of several specific genes, which includes PLAU, IGFBP3, FOLR1, B4GALT1 and COL1A1. Of particular interest to us was a gene cluster, "secreted proteins", that was up-regulated after radiation exposure. Seven up-regulated genes of this gene cluster fall within the chemokine/cytokine gene cluster, namely, CXCL1, CXCL2, IL6, IL11, IL8, IL24 and TGFbeta2. In microarray studies, the radiation-induced up-regulated expression of some these genes encoding cytokine/chemokine proteins was significantly decreased by SeM treatment. For IL8, TGFbeta2, CXCL1 and CXCL2, these observations were validated by qPCR techniques. It is concluded that SeM can regulate ionizing radiation-induced gene expression and may serve as an effective countermeasure for some of the acute inflammatory/immune responses induced by low-dose HZE-particle radiation.|Astronauts[MESH]|Cell Line[MESH]|Cytokines/*genetics[MESH]|Epithelial Cells/drug effects/*metabolism/*radiation effects[MESH]|Extraterrestrial Environment[MESH]|Gene Expression Profiling[MESH]|Humans[MESH]|Iron/*pharmacology[MESH]|Oligonucleotide Array Sequence Analysis[MESH]|Radiation Dosage[MESH]|Reproducibility of Results[MESH]|Reverse Transcriptase Polymerase Chain Reaction[MESH]|Selenomethionine/pharmacology[MESH]|Thyroid Gland/*cytology[MESH]|Transcriptional Activation/drug effects/*radiation effects[MESH] |