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Fluorescence microscopy below the diffraction limit Patterson GHSemin Cell Dev Biol 2009[Oct]; 20 (8): 886-93Fluorescence imaging with conventional microscopy has experienced numerous advances in almost every limiting factor from sensitivity to speed. But improved resolution beyond the fundamental limitation of light diffraction has been elusive until recent years. Now, techniques are available that surpass this barrier and improve resolution up to 10 times over that of conventional microscopy. This chapter provides an overview of these new "super-resolution" imaging methods.|*Cytological Techniques[MESH]|Animals[MESH]|Developmental Biology[MESH]|Limit of Detection[MESH]|Microscopy, Fluorescence/*methods[MESH] |
