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Techniques used to study the DNA polymerase reaction pathway Joyce CMBiochim Biophys Acta 2010[May]; 1804 (5): 1032-40A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.|*Biological Assay[MESH]|Animals[MESH]|DNA-Directed DNA Polymerase/chemistry/*metabolism[MESH]|DNA/*metabolism[MESH]|Humans[MESH]|Substrate Specificity[MESH] |
