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Quantum dots for live cell and in vivo imaging Walling MA; Novak JA; Shepard JREInt J Mol Sci 2009[Feb]; 10 (2): 441-491In the past few decades, technology has made immeasurable strides to enable visualization, identification, and quantitation in biological systems. Many of these technological advancements are occurring on the nanometer scale, where multiple scientific disciplines are combining to create new materials with enhanced properties. The integration of inorganic synthetic methods with a size reduction to the nano-scale has lead to the creation of a new class of optical reporters, called quantum dots. These semiconductor quantum dot nanocrystals have emerged as an alternative to organic dyes and fluorescent proteins, and are brighter and more stable against photobleaching than standard fluorescent indicators. Quantum dots have tunable optical properties that have proved useful in a wide range of applications from multiplexed analysis such as DNA detection and cell sorting and tracking, to most recently demonstrating promise for in vivo imaging and diagnostics. This review provides an in-depth discussion of past, present, and future trends in quantum dot use with an emphasis on in vivo imaging and its related applications.|*Quantum Dots[MESH]|Animals[MESH]|Humans[MESH]|Microscopy/*methods[MESH]|Molecular Imaging/instrumentation/*methods/trends[MESH]|Nanoparticles/chemistry[MESH] |
