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lüll Real-time DNA microarrays: reality check Chagovetz A; Blair SBiochem Soc Trans 2009[Apr]; 37 (Pt 2): 471-5DNA microarrays are plagued with inconsistent quantifications and false-positive results. Using established mechanisms of surface reactions, we argue that these problems are inherent to the current technology. In particular, the problem of multiplex non-equilibrium reactions cannot be resolved within the framework of the existing paradigm. We discuss the advantages and limitations of changing the paradigm to real-time data acquisition similar to real-time PCR methodology. Our analysis suggests that the fundamental problem of multiplex reactions is not resolved by the real-time approach itself. However, by introducing new detection chemistries and analysis approaches, it is possible to extract target-specific quantitative information from real-time microarray data. The possible scope of applications for real-time microarrays is discussed.|*Oligonucleotide Array Sequence Analysis/instrumentation/methods[MESH]|Algorithms[MESH]|Computer Simulation[MESH]|DNA/genetics[MESH]|Genomics[MESH] |