suck pdf from google scholar
unlimited free pdf from europmc19019961    free
PDF from PMC    free
html from PMC    free
PDF vom PMID19019961 :   free
suck pdf from library genesis
English Wikipedia

Nephropedia Template TP (

Twit Text


  • DeepDyve
  • Pubget Overpricing


  • lüll
  • Novel less-abundant viral microRNAs encoded by herpes simplex virus 2 latency-associated transcript and their roles in regulating ICP34 5 and ICP0 mRNAs
  • Tang S; Patel A; Krause PR
  • J Virol 2009[Feb]; 83 (3): 1433-42
  • We recently identified an acutely and latently expressed viral microRNA (miRNA), miR-I, encoded by herpes simplex virus 2 (HSV-2) latency-associated transcript (LAT) through small RNA cloning and two miRNAs encoded by HSV-1 LAT through prediction. We now report the use of high-throughput sequencing technology to identify two additional relatively less-abundant viral miRNAs, miR-II and miR-III, encoded by HSV-2 LAT exon 2. miR-II includes two miRNAs, miR-II-5p and miR-II-3p, which are processed from the same miRNA precursor. miR-II and miR-III map antisense to the 5' untranslated region of ICP34.5 and to the coding region of ICP0 exon 3, respectively. These novel miRNAs are conserved in different HSV-2 strains, and their presence in infected- and transfected-cell cultures was confirmed by Northern hybridization. All three HSV-2 LAT-encoded miRNAs map to genome locations similar to those of three out of four identified HSV-1 LAT-encoded miRNAs, but the sequences of these miRNAs are not conserved. The expression of LAT-encoded miRNAs is negatively regulated by ICP4, the major viral transactivator. We further show that, similar to miR-I, miR-II is able to efficiently silence the expression of ICP34.5, a key viral neurovirulence factor, and that miR-III is able to silence the expression of ICP0, a key viral transactivator. All these data suggest that LAT sequences likely contribute to HSV latency and reactivation through tight control of these LAT-encoded miRNAs and their viral targets.
  • |Base Sequence[MESH]
  • |Blotting, Northern[MESH]
  • |Cell Line[MESH]
  • |DNA Probes[MESH]
  • |Gene Expression Regulation, Viral/*genetics[MESH]
  • |Humans[MESH]
  • |Immediate-Early Proteins/*genetics[MESH]
  • |MicroRNAs/*genetics[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |Promoter Regions, Genetic[MESH]
  • |RNA, Messenger/*genetics[MESH]
  • |RNA, Viral/*genetics[MESH]
  • |Transcription, Genetic[MESH]
  • |Ubiquitin-Protein Ligases/*genetics[MESH]
  • |Viral Proteins/*genetics[MESH]





  • *{{pmid19019961}}
    *<b>[http://www.kidney.de/mlpefetch.php?search=19019961 Novel less-abundant viral microRNAs encoded by herpes simplex virus 2 latency-associated transcript and their roles in regulating ICP34 5 and ICP0 mRNAs ]</b> J Virol 2009; 83(3) ; 1433-42 Tang S; Patel A; Krause PR

        *19019961*

    Nephropedia PMID record

    Deutsche Wikipedia - im Artikel

    Hier den unten stehenden Textblock hineinkopieren

    J Virol

    1433 3.83 2009