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RNA-Seq: a revolutionary tool for transcriptomics Wang Z; Gerstein M; Snyder MNat Rev Genet 2009[Jan]; 10 (1): 57-63RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.|*Transcription, Genetic[MESH]|Animals[MESH]|Base Sequence[MESH]|Chromosome Mapping[MESH]|Exons[MESH]|Gene Expression Profiling/*methods[MESH]|Humans[MESH]|Models, Genetic[MESH]|Molecular Sequence Data[MESH]|RNA/*analysis[MESH]|Sequence Analysis, RNA/*methods[MESH] |
