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lüll A microfluidic device with groove patterns for studying cellular behavior Chung BG; Manbachi A; Khademhosseini AJ Vis Exp 2007[]; ä (7): 270We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 microm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37 degrees C, 5% CO(2)). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.|*Cell Physiological Phenomena[MESH]|Animals[MESH]|Annexin A5/metabolism[MESH]|Apoptosis/physiology[MESH]|Cells/drug effects/metabolism[MESH]|Equipment Design[MESH]|Hydrogen Peroxide/pharmacology[MESH]|Mice[MESH]|Microfluidic Analytical Techniques/*instrumentation[MESH]|NIH 3T3 Cells[MESH]|Oxidants/pharmacology[MESH]|Propidium/metabolism[MESH] |