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lüll The potential and challenges of nanopore sequencing Branton D; Deamer DW; Marziali A; Bayley H; Benner SA; Butler T; Di Ventra M; Garaj S; Hibbs A; Huang X; Jovanovich SB; Krstic PS; Lindsay S; Ling XS; Mastrangelo CH; Meller A; Oliver JS; Pershin YV; Ramsey JM; Riehn R; Soni GV; Tabard-Cossa V; Wanunu M; Wiggin M; Schloss JANat Biotechnol 2008[Oct]; 26 (10): 1146-53A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for approximately $1,000 in approximately 24 h.|*Forecasting[MESH]|Chromosome Mapping/*trends[MESH]|DNA/chemistry/*genetics[MESH]|Genomics/trends[MESH]|Nanostructures/*chemistry/ultrastructure[MESH]|Nanotechnology/*trends[MESH]|Sequence Alignment/*trends[MESH]|Sequence Analysis, DNA/*trends[MESH] |