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Use of CD137 to study the full repertoire of CD8+ T cells without the need to know epitope specificities Wolfl M; Kuball J; Eyrich M; Schlegel PG; Greenberg PDCytometry A 2008[Nov]; 73 (11): 1043-9CD137 (4-1BB) is a member of the TNFR-family with costimulatory function, triggering prosurvival signals in activated T-cells. Upregulation of CD137 upon stimulation allows identifying and isolating live, human antigen-specific CD8+ T-cells of all phenotypes, and therefore provides a comprehensive detection method. Furthermore responses against antigen mixtures can be easily detected, enabling antigen discovery in a stepwise deconvoluting approach. In this article, we will discuss various aspects of this methodology, including potential pitfalls as well as a variety of applications, as illustrated by examples from our laboratory.|CD8-Positive T-Lymphocytes/*immunology[MESH]|Epitopes/*immunology[MESH]|Humans[MESH]|Interferon-gamma/metabolism[MESH]|Lymphocyte Activation[MESH]|Lymphocyte Depletion[MESH]|Tumor Necrosis Factor Receptor Superfamily, Member 9/*immunology[MESH] |