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Determination of protein structures--a series of fortunate events Chruszcz M; Wlodawer A; Minor WBiophys J 2008[Jul]; 95 (1): 1-9Determination of a macromolecular structure using x-ray diffraction is a multistep process that involves a plethora of techniques involving molecular biology, bioinformatics, and physical sciences. Counterintuitively, the success of any or all individual steps does not guarantee the success of the overall process. This review examines the difficulties presented by each step on the path from a gene to the final publication, together with certain lucky (or unlucky) circumstances that can affect the velocity along that path.|*Models, Chemical[MESH]|*Models, Molecular[MESH]|Computer Simulation[MESH]|Crystallography/*methods[MESH]|Protein Conformation[MESH]|Proteins/*chemistry/*ultrastructure[MESH] |
