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lüll Implications of HLA sequence-based typing in transplantation Shankarkumar U; Pawar A; Ghosh KJ Postgrad Med 2008[Jan]; 54 (1): 41-4Serology-based conventional microlymphocytotoxicity HLA typing method, which has been regarded as the gold standard in organ and hematopoietic stem cell transplantation, has been replaced now by DNA-based typing. Many laboratories all over the world have already switched over to molecular methods. Microlymphocytotoxicity-based tissue typing was done using commercial sera, while the molecular typing by genomic DNA based. DNA quality and its quantity obtained using various DNA extraction protocols was found to be an important factor in the molecular method of tissue typing in transplant outcome. Many polymerase chain reaction-based molecular techniques have been adopted with far reaching clinical outcome. The sequence-based typing (SBT) has been the ultimate technique, which has been of the highest reliability in defining the HLA alleles. The nonavailability of specific HLA antisera from native populations, large number of blank alleles yet to be defined and comparable low resolution of HLA alleles in SSP or SSOP technique, suggests that highly refined DNA-based methods like SBT should be used as an adjunct to HLA serology and/or low/intermediate/high resolution HLA typing in order to achieve a better transplant outcome.|Base Sequence/*genetics[MESH]|DNA/analysis[MESH]|HLA Antigens/*genetics/immunology[MESH]|Histocompatibility Testing/*methods[MESH]|Humans[MESH]|Polymerase Chain Reaction/methods[MESH]|Sequence Analysis, DNA/methods[MESH]|Serologic Tests/methods[MESH]|Transplantation Immunology/*genetics/immunology[MESH] |