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lüll Class II major histocompatibility complex tetramer staining: progress, problems, and prospects Vollers SS; Stern LJImmunology 2008[Mar]; 123 (3): 305-13The use of major histocompatibility complex (MHC) tetramers in the detection and analysis of antigen-specific T cells has become more widespread since its introduction 11 years ago. Early challenges in the application of tetramer staining to CD4+ T cells centred around difficulties in the expression of various class II MHC allelic variants and the detection of low-frequency T cells in mixed populations. As many of the technical obstacles to class II MHC tetramer staining have been overcome, the focus has returned to uncertainties concerning how oligomer valency and T-cell receptor/MHC affinity affect tetramer binding. Such issues have become more important with an increase in the number of studies relying on direct ex vivo analysis of antigen-specific CD4+ T cells. In this review we discuss which problems in class II MHC tetramer staining have been solved to date, and which matters remain to be considered.|Antibody Affinity[MESH]|CD4-Positive T-Lymphocytes/*immunology[MESH]|Flow Cytometry/methods[MESH]|Histocompatibility Antigens Class II/*analysis/immunology[MESH]|Humans[MESH]|Staining and Labeling/methods[MESH] |