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Electron cryotomography Murphy GE; Jensen GJBiotechniques 2007[Oct]; 43 (4): 413, 415, 417 passimElectron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.|Biotechnology/instrumentation/methods/trends[MESH]|Cryoelectron Microscopy/instrumentation/*methods/*trends[MESH]|Image Enhancement/instrumentation/*methods[MESH]|Imaging, Three-Dimensional/instrumentation/*methods/*trends[MESH]|Tomography/instrumentation/*methods/*trends[MESH] |