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  • Laser capture sampling and analytical issues in proteomics
  • Gutstein HB; Morris JS
  • Expert Rev Proteomics 2007[Oct]; 4 (5): 627-37
  • Proteomics holds the promise of evaluating global changes in protein expression and post-translational modification in response to environmental stimuli. However, difficulties in achieving cellular anatomic resolution and extracting specific types of proteins from cells have limited the efficacy of these techniques. Laser capture microdissection has provided a solution to the problem of anatomical resolution in tissues. New extraction methodologies have expanded the range of proteins identified in subsequent analyses. This review will examine the application of laser capture microdissection to proteomic tissue sampling, and subsequent extraction of these samples for differential expression analysis. Statistical and other quantitative issues important for the analysis of the highly complex datasets generated are also reviewed.
  • |*Lasers[MESH]
  • |Animals[MESH]
  • |Humans[MESH]
  • |Microdissection[MESH]
  • |Proteins/isolation & purification[MESH]
  • |Proteomics/*methods/statistics & numerical data[MESH]

  • *{{pmid17941818}}
    *<b>[ Laser capture sampling and analytical issues in proteomics ]</b> Expert Rev Proteomics 2007; 4(5) ; 627-37 Gutstein HB; Morris JS


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    Expert Rev Proteomics

    627 5.4 2007