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Vascular development in embryoid bodies: quantification of transgenic intervention and antiangiogenic treatment Evans AL; Bryant J; Skepper J; Smith SK; Print CG; Charnock-Jones DSAngiogenesis 2007[]; 10 (3): 217-26It has become increasingly clear that the investigation of vascular development is best considered in the context of a whole tissue environment since in vivo endothelial cells interact closely with other cell types. Murine embryoid bodies have been used as a model for the early development of a vascular network and are amenable to genetic manipulation and treatment with soluble modulators. However, quantifying morphological changes in these complex three-dimensional structures is challenging. In this paper we describe protocols to culture embryoid bodies on a large scale to study vascular development together with methods to quantify changes seen when antiangiogenic agents or endothelial cell-specific transgenes are introduced.|Angiogenesis Inhibitors/*pharmacology[MESH]|Animals[MESH]|Animals, Genetically Modified[MESH]|Berberine/*pharmacology[MESH]|Bioreactors[MESH]|Cell Culture Techniques[MESH]|Cell Differentiation[MESH]|Cells, Cultured[MESH]|Culture Media/chemistry[MESH]|Embryo, Mammalian/*physiology[MESH]|Embryonic Stem Cells/*cytology[MESH]|Endothelium, Vascular/cytology/*embryology[MESH]|Fluorescent Antibody Technique, Direct[MESH]|Fluorescent Dyes[MESH]|Immunohistochemistry[MESH]|Indoles[MESH]|Mice[MESH]|Neovascularization, Physiologic/drug effects/*physiology[MESH]|Platelet Endothelial Cell Adhesion Molecule-1/metabolism[MESH]|Time Factors[MESH]|Transgenes[MESH] |
