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lüll Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two-substrate reactions Shipovskov S; Reimann CTAnalyst 2007[May]; 132 (5): 397-402The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry (ESI-MS) for monitoring the course of enzyme-substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy (EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems.|Biomarkers/analysis[MESH]|Catalysis[MESH]|Clinical Enzyme Tests[MESH]|Enzymes/*analysis[MESH]|Humans[MESH]|Models, Molecular[MESH]|Spectrometry, Mass, Electrospray Ionization/*methods[MESH]|Substrate Specificity[MESH] |