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lüll Technologies of sleep research Deboer TCell Mol Life Sci 2007[May]; 64 (10): 1227-35Sleep is investigated in many different ways, many different species and under many different circumstances. Modern sleep research is a multidisciplinary venture. Therefore, this review cannot give a complete overview of all techniques used in sleep research and sleep medicine. What it will try to do is to give an overview of widely applied techniques and exciting new developments. Electroencephalography has been the backbone of sleep research and sleep medicine since its first application in the 1930s. The electroencephalogram is still used but now combined with many different techniques monitoring body and brain temperature, changes in brain and blood chemistry, or changes in brain functioning. Animal research has been very important for progress in sleep research and sleep medicine. It provides opportunities to investigate the sleeping brain in ways not possible in healthy volunteers. Progress in genomics has brought new insights in sleep regulation, the best example being the discovery of hypocretin/orexin deficiency as the cause of narcolepsy. Gene manipulation holds great promise for the future since it is possible not only to investigate the functions of different genes under normal conditions, but also to mimic human pathology in much greater detail.|*Research Design[MESH]|Animals[MESH]|Body Temperature/physiology[MESH]|Brain/physiology[MESH]|Circadian Rhythm/physiology[MESH]|Electroencephalography[MESH]|Electrophysiology[MESH]|Humans[MESH]|Sleep/*physiology[MESH] |