Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
 
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve
Pubget Overpricing |  
lüll
Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics Ghosh I; Stains CI; Ooi AT; Segal DJMol Biosyst 2006[Nov]; 2 (11): 551-60Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.|*Zinc Fingers[MESH]|Amino Acid Sequence[MESH]|Animals[MESH]|Base Pairing[MESH]|Cross-Linking Reagents[MESH]|DNA-Binding Proteins/chemistry/genetics[MESH]|DNA/*chemistry[MESH]|Dinucleoside Phosphates[MESH]|Humans[MESH]|Molecular Sequence Data[MESH]|Nylons/*chemistry[MESH]|Protein Engineering/*methods[MESH]|Protein Structure, Tertiary[MESH]|Structure-Activity Relationship[MESH] |
