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lüll Colloidal lithography and current fabrication techniques producing in-plane nanotopography for biological applications Wood MAJ R Soc Interface 2007[Feb]; 4 (12): 1-17Substrate topography plays a vital role in cell and tissue structure and function in situ, where nanometric features, for example, the detail on single collagen fibrils, influence cell behaviour and resultant tissue formation. In vitro investigations demonstrate that nanotopography can be used to control cell reactions to a material surface, indicating its potential application in tissue engineering and implant fabrication. Developments in the catalyst, optical, medical and electronics industries have resulted in the production of nanopatterned surfaces using a variety of methods. The general protocols for nanomanufacturing require high resolution and low cost for fabricating devices. With respect to biological investigations, nanotopographies should occur across a large surface area (ensuring repeatability of experiments and patterning of implant surfaces), be reproducible (allowing for consistency in experiments), and preferably, accessible (limiting the requirement for specialist equipment). Colloidal lithography techniques fit these criteria, where nanoparticles can be utilized in combination with a functionalized substrate to produce in-plane nanotopographies. Subsequent lithographic processing of colloidal substrates utilizing, for example, reactive ion etching allows the production of modified colloidal-derived nanotopographies. In addition to two-dimensional in-plane nanofabrication, functionalized structures can be dip coated in colloidal sols, imparting nanotopographical cues to cells within a three-dimensional environment.|Biocompatible Materials/*chemistry[MESH]|Biotechnology/*methods[MESH]|Cell Culture Techniques/instrumentation/*methods[MESH]|Colloids/*chemistry[MESH]|Nanostructures/*chemistry/*ultrastructure[MESH]|Photography/methods[MESH]|Tissue Engineering/instrumentation/*methods[MESH] |