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lüll A dynamic model for replication protein A (RPA) function in DNA processing pathways Fanning E; Klimovich V; Nager ARNucleic Acids Res 2006[]; 34 (15): 4126-37Processing of DNA in replication, repair and recombination pathways in cells of all organisms requires the participation of at least one major single-stranded DNA (ssDNA)-binding protein. This protein protects ssDNA from nucleolytic damage, prevents hairpin formation and blocks DNA reannealing until the processing pathway is successfully completed. Many ssDNA-binding proteins interact physically and functionally with a variety of other DNA processing proteins. These interactions are thought to temporally order and guide the parade of proteins that 'trade places' on the ssDNA, a model known as 'hand-off', as the processing pathway progresses. How this hand-off mechanism works remains poorly understood. Recent studies of the conserved eukaryotic ssDNA-binding protein replication protein A (RPA) suggest a novel mechanism by which proteins may trade places on ssDNA by binding to RPA and mediating conformation changes that alter the ssDNA-binding properties of RPA. This article reviews the structure and function of RPA, summarizes recent studies of RPA in DNA replication and other DNA processing pathways, and proposes a general model for the role of RPA in protein-mediated hand-off.|DNA Replication/*physiology[MESH]|DNA, Single-Stranded/metabolism[MESH]|DNA-Binding Proteins/*physiology[MESH]|DNA/metabolism[MESH]|Models, Biological[MESH]|Protein Conformation[MESH]|Replication Protein A/chemistry/*physiology[MESH]|Saccharomyces cerevisiae/*genetics[MESH] |