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lüll Phosphoproteomics toolbox: computational biology, protein chemistry and mass spectrometry Hjerrild M; Gammeltoft SFEBS Lett 2006[Sep]; 580 (20): 4764-70Protein phosphorylation is important for regulation of most biological functions and up to 50% of all proteins are thought to be modified by protein kinases. Increased knowledge about potential phosphorylation of a protein may increase our understanding of the molecular processes in which it takes part. Despite the importance of protein phosphorylation, identification of phosphoproteins and localization of phosphorylation sites is still a major challenge in proteomics. However, high-throughput methods for identification of phosphoproteins are being developed, in particular within the fields of bioinformatics and mass spectrometry. In this review, we present a toolbox of current technology applied in phosphoproteomics including computational prediction, chemical approaches and mass spectrometry-based analysis, and propose an integrated strategy for experimental phosphoproteomics.|*Computational Biology/methods[MESH]|*Mass Spectrometry/methods[MESH]|*Proteomics/methods[MESH]|Databases, Factual[MESH]|Humans[MESH]|Internet[MESH]|Phosphorylation[MESH]|Proteins/*chemistry[MESH]|Proteome/analysis[MESH] |