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lüll Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells: an insight into molecular changes Murphy EF; Jewell C; Hooiveld GJ; Muller M; Cashman KDProstaglandins Leukot Essent Fatty Acids 2006[May]; 74 (5): 295-301Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P<0.001-0.01) and claudin 1 down-regulated (2.5-fold; P<0.05) by trans-10, cis-12 CLA, which may underpin its effects on tight-junction function and paracellular Ca transport. On the other hand, expression of key genes involved in transcellular Ca transport (CaT1, ECaC1, calbindin D(9k), vitamin D receptor and PMCA) were unaffected by trans-10, cis-12 CLA. The mechanism by which CLA enhances transcellular Ca transport remains unclear.|Biological Transport/drug effects[MESH]|Caco-2 Cells[MESH]|Calcium Channels/metabolism[MESH]|Calcium/*metabolism[MESH]|Cell Membrane Permeability/drug effects[MESH]|Claudin-1[MESH]|Claudin-4[MESH]|Gene Expression/drug effects[MESH]|Humans[MESH]|Intestinal Mucosa/cytology/*metabolism[MESH]|Linoleic Acids, Conjugated/*pharmacology[MESH]|Membrane Proteins/genetics[MESH]|Occludin[MESH]|Oligonucleotide Array Sequence Analysis/methods[MESH]|Tight Junctions/drug effects/genetics/metabolism[MESH] |