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Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn, New York: epidemiology and recommendations for detection Bratu S; Mooty M; Nichani S; Landman D; Gullans C; Pettinato B; Karumudi U; Tolaney P; Quale JAntimicrob Agents Chemother 2005[Jul]; 49 (7): 3018-20Among 257 isolates of Klebsiella pneumoniae collected in Brooklyn, NY, 24% were found to possess bla(KPC). Clinical microbiology laboratories that used automated broth microdilution systems reported 15% of the KPC-possessing isolates as susceptible to imipenem. The imipenem MIC was found to be markedly affected by the inoculum. For accurate detection of KPC-possessing K. pneumoniae, particular attention should be paid to proper inoculum preparation for broth-based susceptibility methods. In addition, using ertapenem or meropenem for class reporting of carbapenem susceptibility will improve detection.|Anti-Bacterial Agents/*pharmacology[MESH]|Carbapenems/*pharmacology[MESH]|Health Planning Guidelines[MESH]|Humans[MESH]|Klebsiella Infections/*epidemiology/microbiology[MESH]|Klebsiella pneumoniae/*drug effects/enzymology/genetics[MESH]|Laboratories/standards[MESH]|Microbial Sensitivity Tests/methods/standards[MESH]|Microbiology[MESH]|New York City/epidemiology[MESH]|beta-Lactam Resistance[MESH]|beta-Lactamases/genetics/*metabolism[MESH] |
