Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
 
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve
Pubget Overpricing |  
lüll
Characterisation of protein microheterogeneity and protein complexes using on-chip immunoaffinity purification-mass spectrometry Schweigert FJBrief Funct Genomic Proteomic 2005[May]; 4 (1): 7-15Post-translational modification of proteins may influence their interactions with other plasma proteins, as well as having an effect on many aspects of the metabolism of the protein, such as receptor binding, tissue uptake, degradation and excretion. Many post-translational modifications occur in a physiological context, while others are specific for certain diseases, which is why they are of diagnostic importance in clinical proteomics. Analytical approaches to the study of post-translational modifications and protein complexes through the combined use of on-chip immunological affinity purification on a surface-enhanced laser desorption/ionisation platform and subsequent mass spectrometry are illustrated in the author's own work relating to plasma transthyretin (TTR) and retinol-binding protein (RBP). In those studies, both the aspects of post-translational modifications of TTR and the formation of a protein complex between TTR and RBP have been discussed. Such aspects are of diagnostic interest in clinical proteomics, especially with regard to the modification of TTR in relation to the occurrence of amyloidotic diseases.|Amyloid/chemistry[MESH]|Blotting, Western[MESH]|Humans[MESH]|Immunoassay/methods[MESH]|Mass Spectrometry/*methods[MESH]|Prealbumin/chemistry[MESH]|Protein Array Analysis/*methods[MESH]|Protein Processing, Post-Translational[MESH]|Proteins/*chemistry[MESH]|Proteomics/*methods[MESH]|Retinol-Binding Proteins, Plasma[MESH]|Retinol-Binding Proteins/chemistry[MESH] |
