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lüll Molecular technique identifies the pathogen responsible for culture negative infective endocarditis Shin GY; Manuel RJ; Ghori S; Brecker S; Breathnach ASHeart 2005[Jun]; 91 (6): e47A case of culture negative endocarditis complicated by immune complex glomerulonephritis and severe aortic regurgitation necessitated aortic valve replacement. Empirical treatment with penicillin and gentamicin according to UK guidelines was started. The pathogen, Streptococcus sanguis, was later identified by polymerase chain reaction amplification and sequencing of bacterial 16S ribosomal RNA. This molecular technique is likely to be of increasing importance in determining the aetiology of culture negative infective endocarditis, thus providing essential treatment and epidemiological information.|Aortic Valve Insufficiency/*microbiology[MESH]|Autoimmune Diseases/complications[MESH]|Endocarditis, Bacterial/*microbiology[MESH]|Glomerulonephritis/complications[MESH]|Humans[MESH]|Male[MESH]|Middle Aged[MESH]|Polymerase Chain Reaction[MESH]|Streptococcal Infections/*diagnosis[MESH]|Streptococcus sanguis/*isolation & purification[MESH] |