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lüll Detection by radioligand assay of antibodies against Borna disease virus in patients with various psychiatric disorders Matsunaga H; Tanaka S; Sasao F; Nishino Y; Takeda M; Tomonaga K; Ikuta K; Amino NClin Diagn Lab Immunol 2005[May]; 12 (5): 671-6Using a radioligand assay, which preserves the natural form of the antigen, antibodies against Borna disease virus nucleoprotein and phosphoprotein were detected in 11 and 19 sera of 171 psychiatric patients, respectively. Compared with results by Western blotting, three and nine sera were concordantly positive, respectively. The four sera showing the highest levels of antibodies by radioligand assay were all negative by Western blotting; however, dilution and inhibition tests supported the positive results. Our results suggest the importance of conformational structure to detect human anti-Borna disease virus antibodies.|Antibodies, Viral/*blood[MESH]|Blotting, Western[MESH]|Borna Disease/*diagnosis[MESH]|Borna disease virus/immunology/*isolation & purification[MESH]|Female[MESH]|Humans[MESH]|Male[MESH]|Mental Disorders/*virology[MESH]|Middle Aged[MESH]|Mood Disorders/virology[MESH]|Radioligand Assay/*standards[MESH]|Schizophrenia/virology[MESH]|Sensitivity and Specificity[MESH]|Viral Proteins/analysis/immunology[MESH] |