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Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis Wang WK; Chen SY; Liu IJ; Chen YC; Chen HL; Yang CF; Chen PJ; Yeh SH; Kao CL; Huang LM; Hsueh PR; Wang JT; Sheng WH; Fang CT; Hung CC; Hsieh SM; Su CP; Chiang WC; Yang JY; Lin JH; Hsieh SC; Hu HP; Chiang YP; Wang JT; Yang PC; Chang SCEmerg Infect Dis 2004[Jul]; 10 (7): 1213-9The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 10(2) to 5.93 x 10(6) copies/mL) and saliva (7.08 x 10(3) to 6.38 x 10(8) copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.|Adult[MESH]|Communicable Diseases, Emerging/diagnosis/virology[MESH]|Epithelial Cells/virology[MESH]|Female[MESH]|Humans[MESH]|Male[MESH]|Middle Aged[MESH]|Pharynx/*virology[MESH]|RNA, Viral/analysis[MESH]|Reverse Transcriptase Polymerase Chain Reaction[MESH]|Saliva/*virology[MESH]|Severe Acute Respiratory Syndrome/*diagnosis/*virology[MESH]|Severe acute respiratory syndrome-related coronavirus/genetics/*isolation & purification[MESH]|Specimen Handling/methods[MESH] |
