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lüll Focusing light on infection in four dimensions Roux P; Munter S; Frischknecht F; Herbomel P; Shorte SLCell Microbiol 2004[Apr]; 6 (4): 333-43The fusion of cell biology with microbiology has bred a new discipline, cellular microbiology, in which the primary aim is to understand host-pathogen interactions at a tissue, cellular and molecular level. In this context, we require techniques allowing us to probe infection in situ and extrapolate quantitative information on its spatiotemporal dynamics. To these ends, fluorescent light-based imaging techniques offer a powerful tool, and the state-of-the-art is defined by paradigms using so-called multidimensional (multi-D) imaging microscopy. Multi-D imaging aims to visualize and quantify biological events through time and space and, more specifically, refers to combinations of: three (3D, volume), four (4D, time) and five (5D, multiwavelength)-dimensional recordings. Successful multi-D imaging depends upon understanding the available technologies and their limitations. This is especially true in the field of microbiology where visualization of infectious/pathogenic activities inside living host systems presents particular technical challenges. Thus, as multi-D imaging rapidly becomes a common bench tool to the cellular microbiologist, this review provides the new user with some of the necessary technical insight required to get the best from these methods.|*Infections/microbiology/parasitology/virology[MESH]|*Light[MESH]|Animals[MESH]|Cell Line[MESH]|Cells, Cultured[MESH]|Fluorescence[MESH]|Host-Parasite Interactions[MESH]|Humans[MESH]|Image Processing, Computer-Assisted/*methods[MESH]|Imaging, Three-Dimensional/instrumentation/*methods[MESH]|Microscopy, Confocal/methods[MESH]|Microscopy, Fluorescence/methods[MESH]|Microscopy/*methods[MESH] |