Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
 
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve
Pubget Overpricing |  
lüll
A role for Ca(v)1 3 in rat intestinal calcium absorption Morgan EL; Mace OJ; Helliwell PA; Affleck J; Kellett GLBiochem Biophys Res Commun 2003[Dec]; 312 (2): 487-93Active Ca(2+) absorption through epithelial Ca(2+) channels TRPV5/6 in duodenum is activated by hyperpolarisation. However, when diet and Ca(2+) are plentiful, digestion products cause depolarisation. We therefore used homology-based PCR from a rat jejunal mucosal cDNA preparation to reveal the presence of the neuroendocrine L-type isoform Ca(v)1.3alpha(1). Immunocytochemical labelling and immunoblotting localised Ca(v)1.3 alpha(1) protein in apical membrane from proximal jejunum to mid ileum. Perfusion studies in vivo with 1.25 mM luminal Ca(2+) revealed L-type channel activity. Inhibition of glucose absorption with phloridzin strongly inhibited 45Ca(2+) absorption; absorption was inhibited by nifedipine and Mg(2+) and activated by Bay K 8644, none of which affect TRPV5/6. At 10mM Ca(2+), nifedipine inhibited 45Ca(2+) absorption with a time course similar to that at 1.25 mM Ca(2+): absorption was therefore channel-mediated rather than paracellular. We suggest that in times of dietary sufficiency, Ca(v)1.3 may mediate a significant route of Ca(2+) absorption into the body.|3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology[MESH]|Absorption[MESH]|Animals[MESH]|Calcium Channels[MESH]|Calcium Channels, L-Type/drug effects/*metabolism[MESH]|Calcium/*pharmacokinetics[MESH]|Culture Techniques[MESH]|Intestinal Mucosa/*metabolism[MESH]|Ion Channel Gating/drug effects/physiology[MESH]|Jejunum/metabolism[MESH]|Magnesium/pharmacology[MESH]|Male[MESH]|Nifedipine/pharmacology[MESH]|Phlorhizin/pharmacology[MESH]|Rats[MESH]|Rats, Wistar[MESH] |
