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lüll IL-4 from Th2-type cells suppresses induction of delayed-type hypersensitivity elicited shortly after immunization Jacysyn JF; Abrahamsohn IA; Macedo MSImmunol Cell Biol 2003[Dec]; 81 (6): 424-30The pure delayed-type hypersensitivity reaction obtained in 4-day ovalbumin-sensitized mice after antigen challenge in the footpad was abrogated by transfer of in vitro expanded, antigen-specific lymphoblasts derived from ovalbumin-hyperimmunized donors (high antibody producers), 12 h before immunization. This effect was specific inasmuch as Trypanosoma cruzi-specific blasts derived from Tc-Ag-hyperimmunized mice did not inhibit delayed-type hypersensitivity in ovalbumin-immunized recipients. The ovalbumin-specific blasts displayed a Th2 cytokine profile, secreting IL-4 and IL-10 upon restimulation in vitro with ovalbumin, but not IFN-gamma or IL-2. In addition, recipients of such cells produced much more IgG1 and IgE antibodies. When the frequency of T-cell blasts was enriched among these cells, transfer of four million cells was enough to prevent the induction of delayed-type hypersensitivity. Neutralization of IL-4 alone just before cell transfer not only restored the delayed-type hyper-sensitivity reaction, but also maintained it in a plateau for at least 72 h after challenge. Recipients treated in this way also showed a shift back towards a Th1 phenotype, indicated by the increase in IL-2, IFN-gamma and IL-12 synthesis. No synergistic action was observed when IL-4 and IL-10 were concomitantly neutralized. These results indicate that activation of Ag-specific Th2 cells early in the course of the immune response to a protein antigen provides an immunological environment rich in IL-4, thus leading to the inhibition of cell-mediated immunity.|Animals[MESH]|Antibodies, Monoclonal/pharmacology[MESH]|Cells, Cultured[MESH]|Female[MESH]|Hypersensitivity, Delayed/*immunology/therapy[MESH]|Immunization[MESH]|Interleukin-10/antagonists & inhibitors[MESH]|Interleukin-4/antagonists & inhibitors/*physiology[MESH]|Lymphocyte Activation/drug effects/*immunology[MESH]|Mice[MESH]|Mice, Inbred DBA[MESH]|Ovalbumin/administration & dosage/immunology[MESH]|Th2 Cells/*immunology/transplantation[MESH] |