suck pdf from google scholar
PDF vom PMID14625201 :   free
suck pdf from library genesis
English Wikipedia

Nephropedia Template TP (

Twit Text


  • DeepDyve
  • Pubget Overpricing


  • lüll
  • Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport
  • Diepens RJ; den Dekker E; Bens M; Weidema AF; Vandewalle A; Bindels RJ; Hoenderop JG
  • Am J Physiol Renal Physiol 2004[Mar]; 286 (3): F483-9
  • To unravel the molecular regulation of renal transcellular Ca(2+) transport, a murine distal convoluted tubule (mpkDCT) cell line derived from distal convoluted tubules (DCT) microdissected from a SV-PK/Tag transgenic mouse was characterized. This cell line originated from DCT only, as mRNA encoding for the DCT marker thiazide-sensitive Na(+)/Cl(-) cotransporter was expressed, whereas mRNA encoding for the connecting tubule and collecting duct marker aquaporin-2 was not detected, as determined by reverse-transcriptase PCR. mpkDCT cells expressed mRNA encoding the Ca(2+) channels TRPV5 and TRPV6 and other key players necessary for transcellular Ca(2+) transport, i.e., calbindin-D(9k), calbindin-D(28k), plasma membrane Ca(2+)-ATPase isoform 1b, and Na(+)/Ca(2+) exchanger 1. Primary cultures of DCT cells exhibited net transcellular Ca(2+) transport of 0.4 +/- 0.1 nmol.h(-1).cm(-2), whereas net transcellular Ca(2+) transport across mpkDCT cells was significantly higher at 2.4 +/- 0.4 nmol.h(-1).cm(-2). Transcellular Ca(2+) transport across mpkDCT cells was completely inhibited by ruthenium red, an inhibitor of TRPV5 and TRPV6, but not by the voltage-operated Ca(2+) channel inhibitors felodipine and verapamil. With the use of patch-clamp analysis, the IC(50) of ruthenium red on Na(+) currents was between the values measured for TRPV5- and TRPV6-expressing HEK 293 cells, suggesting that TRPV5 and/or TRPV6 is possibly active in mpkDCT cells. Forskolin in combination with IBMX, 1,25-dihydroxyvitamin D(3), and 1-deamino-8-d-arginine vasopressin increased transcellular Ca(2+) transport, whereas PMA and parathyroid hormone had no significant effect. In conclusion, the murine mpkDCT cell line provides a unique cell model in which to study the molecular regulation of transcellular Ca(2+) transport in the kidney in vitro.
  • |*Cell Line[MESH]
  • |Animals[MESH]
  • |Calcitriol/pharmacology[MESH]
  • |Calcium/*metabolism[MESH]
  • |Cyclic AMP/metabolism[MESH]
  • |Electric Conductivity[MESH]
  • |Female[MESH]
  • |Ion Transport[MESH]
  • |Kidney Tubules, Distal/*cytology/drug effects/*metabolism[MESH]
  • |Mice[MESH]
  • |Mice, Inbred C57BL[MESH]
  • |RNA, Messenger/metabolism[MESH]
  • |Ruthenium Red/pharmacology[MESH]





  • *{{pmid14625201}}
    *<b>[http://www.kidney.de/mlpefetch.php?search=14625201 Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport ]</b> Am J Physiol Renal Physiol 2004; 286(3) ; F483-9 Diepens RJ; den Dekker E; Bens M; Weidema AF; Vandewalle A; Bindels RJ; Hoenderop JG

        *14625201*

    Nephropedia PMID record

    Deutsche Wikipedia - im Artikel

    Hier den unten stehenden Textblock hineinkopieren

    Am J Physiol Renal Physiol

    F483 3.286 2004