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lüll Vimentin-positive cells in the epithelium of rabbit ileal villi represent cup cells but not M-cells Ramirez C; Gebert AJ Histochem Cytochem 2003[Nov]; 51 (11): 1533-44Membranous (M)-cells are specialized epithelial cells of the Peyer's patch domes that transport antigens from the intestinal lumen to the lymphoid tissue. Vimentin is a reliable marker for M-cells in rabbits. Using immunohistochemistry (IHC), a subpopulation of epithelial cells has recently been identified in ordinary rabbit ileal villi, which are vimentin-positive and share morphological characteristics with the M-cells of the domes. To test the hypothesis that these cells represent M-cells outside the organized lymphoid tissue, lectin labeling and tracer uptake experiments were performed. Lectins specific for N-acetyl-glucosamine oligomers selectively bound to the vimentin-positive villous cells but not to M-cells in the domes. Microbeads instilled into the ileal lumen were taken up by M-cells within 45 min but not by the vimentin-positive cells in the villi. Lectin-gold labeling on ultrathin sections revealed that the lectin binding sites were located in the brush border and in vesicles in the apical cytoplasm. The vimentin/lectin-positive cells shared ultrastructural characteristics with the so-called "cup cells." We conclude (a) that the vimentin-positive cells in ordinary villi represent cup cells but not M-cells, (b) that they are readily detectable by (GlucNAc)(N)-specific lectins, and (c) that they do not transcytose experimental tracers. Although the specific function of cup cells is still obscure, they most probably represent a cell type distinct from M-cells of the domes with respect to both function and expression of the two new markers.|Animals[MESH]|Ileum/*cytology/metabolism/ultrastructure[MESH]|Immunohistochemistry[MESH]|Intestinal Mucosa/*cytology/metabolism/ultrastructure[MESH]|Lectins/metabolism[MESH]|Microscopy, Confocal[MESH]|Microscopy, Fluorescence[MESH]|Microvilli/metabolism/ultrastructure[MESH]|Rabbits[MESH]|Vimentin/*metabolism[MESH] |