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Active borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphoprotein ratio but no viral X protein Schneider U; Naegele M; Staeheli P; Schwemmle MJ Virol 2003[Nov]; 77 (21): 11781-9Analysis of the composition and regulation of the Borna disease virus (BDV) polymerase complex has so far been limited by the lack of a functional assay. To establish such an assay on the basis of an artificial minigenome, we constructed expression vectors encoding either nucleoprotein (N), phosphoprotein (P), X protein, or polymerase (L) of BDV under the control of the chicken beta-actin promoter. A Flag-tagged version of L colocalized with virus-encoded N and P in characteristic nuclear dots of BDV-infected cells and increased viral N-protein levels in persistently infected Vero cells. Vector-driven expression of L, N, and P in BSR-T7 cells together with a negative-sense BDV minigenome carrying a chloramphenicol acetyltransferase (CAT) reporter gene resulted in efficient synthesis of CAT protein. Induction of CAT protein synthesis strongly depended on a 10- to 30-fold molar excess of the N-encoding plasmid over the P-encoding plasmid. Cotransfection of even small amounts of plasmid encoding the viral X protein reduced CAT synthesis to background levels. Thus, the N-to-P stoichiometry seems to play a central role in the regulation of the BDV polymerase complex. Our data further suggest a negative regulatory function for the X protein of BDV.|*Gene Expression Regulation, Viral[MESH]|Animals[MESH]|Borna disease virus/*enzymology/genetics/*metabolism[MESH]|Cell Line[MESH]|Chloramphenicol O-Acetyltransferase/genetics/metabolism[MESH]|Chlorocebus aethiops[MESH]|Genes, Reporter[MESH]|Nucleoproteins/genetics/metabolism[MESH]|Phosphoproteins/genetics/metabolism[MESH]|RNA-Dependent RNA Polymerase/genetics/*metabolism[MESH]|Replicon[MESH]|Transfection[MESH]|Vero Cells[MESH]|Viral Proteins/genetics/*metabolism[MESH]|Virology/methods[MESH] |
