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lüll Two major histocompatibility complex class I-restricted epitopes of the Borna disease virus p10 protein identified by cytotoxic T lymphocytes induced by DNA-based immunization Hashimoto Y; Chen HS; Cunningham C; Malik TH; Lai PKJ Virol 2003[May]; 77 (10): 6076-81Borna disease virus (BDV) infection of Lewis rats is the most studied animal model of Borna disease, an often fatal encephalomyelitis. In this experimental model, BDV-specific CD8(+) cytotoxic T lymphocytes (CTLs) play a prominent role in the immunopathogenesis of infection by the noncytolytic, persistent BDV. Of the six open reading frames of BDV, CTLs to BDV X (p10) and the L-polymerase have never been studied. In this study, we used plasmid immunization to investigate the CTL response to BDV X and N. Plasmid-based immunization was a potent CTL inducer in Lewis rats. Anti-X CTLs were primed by a single injection of the p10 cDNA. Two codominant p10 epitopes, M(1)SSDLRLTLL(10) and T(8)LLELVRRL(16), associated with the RT1.A(l) major histocompatibility complex class I molecules of the Lewis rats, were identified. In addition, immunization with a BDV p40-expressing plasmid confirmed the previously reported RT1.A(l)-restricted A(230)SYAQMTTY(238) peptide as the CTL target for BDV N. In contrast to the CTL responses, plasmid vaccination was a poor inducer of an antibody response to p10. Three injections of a recombinant eukaryotic expression plasmid of BDV p10 were needed to generate a weak anti-p10 immunoglobulin M response. However, the antibody response could be optimized by a protein boost after priming with cDNA.|Amino Acid Sequence[MESH]|Animals[MESH]|Antibodies, Viral/blood[MESH]|Borna Disease/immunology/prevention & control[MESH]|Borna disease virus/*immunology[MESH]|Epitope Mapping[MESH]|Epitopes, T-Lymphocyte/chemistry/*immunology[MESH]|Histocompatibility Antigens Class I/*metabolism[MESH]|Immunization[MESH]|Molecular Sequence Data[MESH]|Plasmids[MESH]|Rats[MESH]|Rats, Inbred Lew[MESH]|T-Lymphocytes, Cytotoxic/*immunology[MESH]|Vaccines, DNA/*immunology[MESH]|Viral Proteins/genetics/*immunology[MESH]|Viral Vaccines/genetics/immunology[MESH] |