free     free     free  :   free English Wikipedia <ref>{{Cite pmid|12660155}}</ref> Nephropedia Template TP ( {{tp|p=12660155|t=Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex |pdf=|usr=|}} Twit Text Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex http://www.kidney.de/pget.php?&tw=1&pmid=12660155 DeepDyve Pubget Overpricing

lüll Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex van de Graaf SF; Hoenderop JG; Gkika D; Lamers D; Prenen J; Rescher U; Gerke V; Staub O; Nilius B; Bindels RJ EMBO J 2003[Apr]; 22 (7): 1478-87 TRPV5 and TRPV6 constitute the Ca(2+) influx pathway in a variety of epithelial cells. Here, we identified S100A10 as the first auxiliary protein of these epithelial Ca(2+) channels using yeast two-hybrid and GST pull-down assays. This S100 protein forms a heterotetrameric complex with annexin 2 and associates specifically with the conserved sequence VATTV located in the C-terminal tail of TRPV5 and TRPV6. Of these five amino acids, the first threonine plays a crucial role since the corresponding mutants (TRPV5 T599A and TRPV6 T600A) exhibited a diminished capacity to bind S100A10, were redistributed to a subplasma membrane area and did not display channel activity. Using GST pull-down and co-immunoprecipitation assays we demonstrated that annexin 2 is part of the TRPV5-S100A10 complex. Furthermore, the S100A10-annexin 2 pair colocalizes with the Ca(2+) channels in TRPV5-expressing renal tubules and TRPV6-expressing duodenal cells. Importantly, downregulation of annexin 2 using annexin 2-specific small interfering RNA inhibited TRPV5 and TRPV6-mediated currents in transfected HEK293 cells. In conclusion, the S100A10-annexin 2 complex plays a crucial role in routing of TRPV5 and TRPV6 to plasma membrane. |*S100 Proteins[MESH] |Animals[MESH] |Annexin A2/metabolism[MESH] |Base Sequence[MESH] |Calcitriol/metabolism[MESH] |Calcium Channels/*metabolism[MESH] |Calcium-Binding Proteins/metabolism[MESH] |DNA Primers[MESH] |Epithelium/metabolism[MESH] |Gene Silencing[MESH] |Immunohistochemistry[MESH] |Mice[MESH] |Protein Binding[MESH] |RNA Interference[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Two-Hybrid System Techniques[MESH]

*{{pmid12660155}}
*<b>[http://www.kidney.de/mlpefetch.php?search=12660155 Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex ]</b> EMBO J 2003; 22(7) ; 1478-87 van de Graaf SF; Hoenderop JG; Gkika D; Lamers D; Prenen J; Rescher U; Gerke V; Staub O; Nilius B; Bindels RJ

    *12660155*

Nephropedia PMID record
{{recpmid|pmid=12660155|ti=Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex |au=van de Graaf SF; Hoenderop JG; Gkika D; Lamers D; Prenen J; Rescher U; Gerke V; Staub O; Nilius B; Bindels RJ|so=EMBO J 2003; 22 (7): 1478-87|ab=TRPV5 and TRPV6 constitute the Ca(2+) influx pathway in a variety of epithelial cells. Here, we identified S100A10 as the first auxiliary protein of these epithelial Ca(2+) channels using yeast two-hybrid and GST pull-down assays. This S100 protein forms a heterotetrameric complex with annexin 2 and associates specifically with the conserved sequence VATTV located in the C-terminal tail of TRPV5 and TRPV6. Of these five amino acids, the first threonine plays a crucial role since the corresponding mutants (TRPV5 T599A and TRPV6 T600A) exhibited a diminished capacity to bind S100A10, were redistributed to a subplasma membrane area and did not display channel activity. Using GST pull-down and co-immunoprecipitation assays we demonstrated that annexin 2 is part of the TRPV5-S100A10 complex. Furthermore, the S100A10-annexin 2 pair colocalizes with the Ca(2+) channels in TRPV5-expressing renal tubules and TRPV6-expressing duodenal cells. Importantly, downregulation of annexin 2 using annexin 2-specific small interfering RNA inhibited TRPV5 and TRPV6-mediated currents in transfected HEK293 cells. In conclusion, the S100A10-annexin 2 complex plays a crucial role in routing of TRPV5 and TRPV6 to plasma membrane.}}
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<ref>{{BibPMID|12660155}}</ref>
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{{BibRecord | Autor = van de Graaf SF; Hoenderop JG; Gkika D; Lamers D; Prenen J; Rescher U; Gerke V; Staub O; Nilius B; Bindels RJ | Titel = Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex | Band = 22 | Nummer = 7 | Jahr = 2003 | Monat = Apr | Sprache = eng | DOI = | Seite = 1478-87 | PMID = 12660155 | Typ= {{{Typ|}}} | Kommentar = {{{Kommentar|}}} | format = {{{format|}}} | record = {{{record|}}} }}

EMBO J