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lüll Identification of the amino terminal subunit of the glycoprotein of Borna disease virus Kiermayer S; Kraus I; Richt JA; Garten W; Eickmann MFEBS Lett 2002[Nov]; 531 (2): 255-8The only surface membrane glycoprotein of Borna disease virus (BDV) is synthesized as a polypeptide with a molecular mass of 57 kDa and N-glycosylated to a precursor glycoprotein (GP) of about 94 kDa. It is processed by the cellular protease furin into the C-terminal membrane-anchored subunit GP-C, also known as gp43, and a presumptive N-terminal subunit GP-N, that is highly glycosylated and has a molecular mass of about 51 kDa. However, up to now the latter remained undetected in BDV-infected material. We describe a novel approach to identify glycan masked linear antigenic epitopes. In the present study, GP-N was identified in BDV-infected cells by a combination of lectin precipitation, enzymatic deglycosylation on blot and immunochemistry using an N-terminal specific antiserum. The GP-N has an apparent molecular mass of 45-50 kDa in its glycosylated form and 27 kDa in its deglycosylated form. N-glycan analysis revealed that the precursor GP contains only mannose-rich N-glycans, whereas GP-N and GP-C contain mannose-rich and complex-type N-glycans.|*Borna disease virus/immunology[MESH]|Animals[MESH]|Cell Line[MESH]|Chlorocebus aethiops[MESH]|Dogs[MESH]|Glycoproteins/analysis/*chemistry/immunology[MESH]|Glycoside Hydrolases[MESH]|Immunoblotting[MESH]|Lectins/metabolism[MESH]|Mannose/analysis[MESH]|Molecular Weight[MESH]|Polysaccharides/chemistry[MESH]|Protein Subunits[MESH]|Vero Cells[MESH]|Viral Proteins/analysis/*chemistry/immunology[MESH] |