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lüll Maxi K+ channel mediates regulatory volume decrease response in a human bronchial epithelial cell line Fernandez-Fernandez JM; Nobles M; Currid A; Vazquez E; Valverde MAAm J Physiol Cell Physiol 2002[Dec]; 283 (6): C1705-14The cell regulatory volume decrease (RVD) response triggered by hypotonic solutions is mainly achieved by the coordinated activity of Cl- and K+ channels. We now describe the molecular nature of the K(+) channels involved in the RVD response of the human bronchial epithelial (HBE) cell line 16HBE14o-. These cells, under isotonic conditions, present a K+ current consistent with the activity of maxi K+ channels, confirmed by RT-PCR and Western blot. Single-channel and whole cell maxi K+ currents were readily and reversibly activated following the exposure of HBE cells to a 28% hypotonic solution. Both maxi K+ current activation and RVD response showed calcium dependency, inhibition by TEA, Ba2+, iberiotoxin, and the cationic channel blocker Gd3+ but were insensitive to clofilium, clotrimazole, and apamin. The presence of the recently cloned swelling-activated, Gd3+-sensitive cation channels (TRPV4, also known as OTRPC4, TRP12, or VR-OAC) was detected by RT-PCR in HBE cells. This channel, TRPV4, which senses changes in volume, might provide the pathway for Ca2+ influx under hypotonic solutions and, consequently, for the activation of maxi K+ channels.|Bronchi/*cytology/drug effects/*metabolism[MESH]|Cell Line[MESH]|Electric Conductivity[MESH]|Epithelial Cells/drug effects/metabolism[MESH]|Humans[MESH]|Hypotonic Solutions/pharmacology[MESH]|Large-Conductance Calcium-Activated Potassium Channels[MESH]|Potassium Channels, Calcium-Activated/*physiology[MESH] |