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lüll CaT1 contributes to the stores-operated calcium current in Jurkat T-lymphocytes Cui J; Bian JS; Kagan A; McDonald TVJ Biol Chem 2002[Dec]; 277 (49): 47175-83T-lymphocyte activation requires sustained Ca(2+) signaling dependent upon capacitative Ca(2+) entry (CCE). The protein(s) that forms the stores-operated Ca(2+) channel (SOCC) responsible for CCE has long been sought but has not been definitively identified. Members of the TRPV family (transient receptor potential superfamily-vanilloid receptor subfamily) of channel genes have been proposed to encode SOCCs responsible for CCE in non-excitable cells. Here we present evidence that a member of the TRPV group, CaT1, is involved in generating I(CRAC), the CCE current that is necessary for T-cell activation. CaT1 is expressed in Jurkat T-lymphocytes. When overexpressed in Jurkat cells, CaT1 produces a Ca(2+) entry current that mimics the endogenous I(CRAC) in its dependence on external Ca(2+), inactivation by elevated concentration of internal Ca(2+), and pharmacological block by capsaicin. Overexpressed CaT1 is partially regulated by the release of internal Ca(2+) stores via thapsigargin or receptor-mediated generation of inositol 1,4,5-trisphosphate. A pore-region mutant of CaT1, TRIA-CaT1, fails to carry Ca(2+) currents and associates with co-expressed wild type CaT1 to functionally suppress permeation of Ca(2+) ions. Expression of the TRIA-CaT1 mutant in Jurkat cells results in suppression of the endogenous I(CRAC). Taken together these results indicate that CaT1 is the channel protein that contributes to T-lymphocyte SOCCs either alone or as a subunit in a heterogeneous channel complex.|Animals[MESH]|CHO Cells[MESH]|Calcium Channels/genetics/*metabolism/physiology[MESH]|Calcium/*metabolism[MESH]|Capsaicin/pharmacology[MESH]|Cloning, Molecular[MESH]|Cricetinae[MESH]|DNA, Complementary/metabolism[MESH]|Genes, Dominant[MESH]|Humans[MESH]|Immunoblotting[MESH]|Inositol 1,4,5-Trisphosphate/metabolism[MESH]|Jurkat Cells[MESH]|Microscopy, Fluorescence[MESH]|Mutation[MESH]|Reverse Transcriptase Polymerase Chain Reaction[MESH]|TRPV Cation Channels[MESH]|Time Factors[MESH]|Transfection[MESH] |