Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Microdissection techniques for cancer analysis Cor A; Vogt N; Malfoy BFolia Biol (Praha) 2002[]; 48 (1): 3-8One difficulty in studying molecular changes of tumours has been the inability to isolate DNA and RNA from a homogeneous cell population. The combination of several new technologies should help overcome these hurdles. Microdissection is a technique for rapid and easy procurement of a pure cellular subpopulation away from its complex tissue milieu. Laser-assisted microdissection has recently been identified as a quick, simple and effective method by which microdissection of complex tissue specimens can be routinely performed for molecular analysis. With the advent of laser microdissection, cDNA libraries can be developed from pure cells obtained directly from stained neoplastic tissue, and microarrays of thousands of genes can now be used to examine gene expression in microdissected tumour tissue samples. This review will concentrate on the application of different microdissection techniques in the area of cancer research.|*Gene Expression Profiling[MESH]|Cell Separation/*methods[MESH]|Cytogenetics/instrumentation/*methods[MESH]|DNA, Complementary/genetics[MESH]|DNA, Neoplasm/analysis[MESH]|Disease Progression[MESH]|Dissection/instrumentation/*methods[MESH]|Humans[MESH]|Micromanipulation/instrumentation/*methods[MESH]|Neoplasm Proteins/analysis[MESH]|Neoplasms/genetics/*pathology[MESH]|Neoplastic Stem Cells/*chemistry[MESH]|Oligonucleotide Array Sequence Analysis[MESH]|RNA, Neoplasm/analysis[MESH] |