Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
free
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
free free
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Biosynthesis of polyketides in heterologous hosts Pfeifer BA; Khosla CMicrobiol Mol Biol Rev 2001[Mar]; 65 (1): 106-18Polyketide natural products show great promise as medicinal agents. Typically the products of microbial secondary biosynthesis, polyketides are synthesized by an evolutionarily related but architecturally diverse family of multifunctional enzymes called polyketide synthases. A principal limitation for fundamental biochemical studies of these modular megasynthases, as well as for their applications in biotechnology, is the challenge associated with manipulating the natural microorganism that produces a polyketide of interest. To ameliorate this limitation, over the past decade several genetically amenable microbes have been developed as heterologous hosts for polyketide biosynthesis. Here we review the state of the art as well as the difficulties associated with heterologous polyketide production. In particular, we focus on two model hosts, Streptomyces coelicolor and Escherichia coli. Future directions for this relatively new but growing technological opportunity are also discussed.|Biotechnology/methods[MESH]|Carrier Proteins/metabolism[MESH]|Escherichia coli/genetics/metabolism[MESH]|Fungi/genetics/metabolism[MESH]|Genetic Engineering/*methods[MESH]|Multienzyme Complexes/genetics/metabolism[MESH]|Plants/genetics/metabolism[MESH]|Protein Processing, Post-Translational[MESH]|Recombinant Proteins/*genetics/*metabolism[MESH]|Streptomyces/genetics/metabolism[MESH]|Substrate Specificity[MESH] |