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Microarrays under the microscope Wildsmith SE; Elcock FJMol Pathol 2001[Feb]; 54 (1): 8-16Microarray technology is a rapidly advancing area, which is gaining popularity in many biological disciplines from drug target identification to predictive toxicology. Over the past few years, there has been a dramatic increase in the number of methods and techniques available for carrying out this form of gene expression analysis. The techniques and associated peripherals, such as slide types, deposition methods, robotics, and scanning equipment, are undergoing constant improvement, helping to drive the technology forward in terms of robustness and ease of use. These rapid developments, combined with the number of options available and the associated hyperbole, can prove daunting for the new user. This review aims to guide the researcher through the various steps of conducting microarray experiments, from initial strategy to analysing the data, with critical examination of the benefits and disadvantages along the way.|DNA, Complementary/genetics[MESH]|Gene Expression Profiling/*methods/trends[MESH]|Humans[MESH]|Image Processing, Computer-Assisted/methods[MESH]|Oligonucleotide Array Sequence Analysis/*methods/trends[MESH] |
