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lüll Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1) Muller D; Hoenderop JG; Meij IC; van den Heuvel LP; Knoers NV; den Hollander AI; Eggert P; Garcia-Nieto V; Claverie-Martin F; Bindels RJGenomics 2000[Jul]; 67 (1): 48-53Functional and morphological analyses indicated that the epithelial Ca2+ channel (ECaC), which was recently cloned from rabbit kidney, exhibits the defining properties for being the gatekeeper in transcellular Ca2+ (re)absorption. Its human homologue provides, therefore, a molecular basis for achieving a better understanding of Ca2+ mal(re)absorption. By applying the RACE technique, the full-length cDNA of human ECaC (HGMW-approved symbol ECAC1) was obtained. It consisted of 2,772 bp with an open reading frame of 2,187 bp encoding a protein of 729 amino acids with a predicted molecular mass of 83 kDa. Phylogenetic analysis indicated that this highly selective Ca2+ channel exhibits a low level of homology (<30%) to other Ca2+ channels, suggesting that it belongs to a new family. hECaC was highly expressed in kidney, small intestine, and pancreas, and less intense expression was detected in testis, prostate, placenta, brain, colon, and rectum. These ECaC-positive tissues also expressed the 1,25-dihydroxyvitamin D3-sensitive calcium-binding proteins, calbindin-D9K and/or calbindin-D28K. The human ECaC gene mapped to chromosome 7q31.1-q31.2. Taken together, the conspicuous colocalization of hECaC and calbindins in organs that are not prime regulators of plasma Ca2+ levels could illustrate new pathways in cellular Ca2+ homeostasis.|Amino Acid Sequence[MESH]|Animals[MESH]|Calbindin 1[MESH]|Calbindins[MESH]|Calcium Channels/chemistry/*genetics/metabolism[MESH]|Cells, Cultured[MESH]|Chromosomes, Human, Pair 7[MESH]|Cloning, Molecular[MESH]|DNA Primers/chemistry[MESH]|Epithelial Cells/*metabolism[MESH]|Humans[MESH]|Molecular Sequence Data[MESH]|Molecular Structure[MESH]|Phylogeny[MESH]|Physical Chromosome Mapping[MESH]|RNA, Messenger/analysis[MESH]|S100 Calcium Binding Protein G/metabolism[MESH]|Sequence Analysis, DNA[MESH]|Sequence Homology, Amino Acid[MESH]|TRPV Cation Channels[MESH]|Tissue Distribution[MESH] |