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Imaging membrane potential with voltage-sensitive dyes Zochowski M; Wachowiak M; Falk CX; Cohen LB; Lam YW; Antic S; Zecevic DBiol Bull 2000[Feb]; 198 (1): 1-21Membrane potential can be measured optically using a variety of molecular probes. These measurements can be useful in studying function at the level of an individual cell, for determining how groups of neurons generate a behavior, and for studying the correlated behavior of populations of neurons. Examples of the three kinds of measurements are presented. The signals obtained from these measurements are generally small. Methodological considerations necessary to optimize the resulting signal-to-noise ratio are discussed.|*Fluorescent Dyes[MESH]|*Membrane Potentials[MESH]|Animals[MESH]|Electric Conductivity[MESH]|Image Processing, Computer-Assisted[MESH]|Neurons/physiology[MESH] |
