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A novel TNF-inducible message with putative growth suppressor function Nadadur SS; Ehrke MJ; Gurtoo HLBiochim Biophys Acta 1999[Dec]; 1489 (2-3): 433-9We report the nucleotide sequence of a novel cDNA and TNF-induced expression of the corresponding message (mRNA) in human fibroblast cells. This message is also expressed in certain human tumor cell lines and is over-expressed in a colon cancer cell line (HT-29). NIH3T3 cells transfected with the antisense construct of the 5'-region of this novel cDNA formed 20-fold more colonies in culture compared to cells transfected with a sense construct of the same region or the sense and the antisense constructs of the central region of this cDNA. This observation suggests a possible growth suppressor function for the gene represented by this cDNA.|3T3 Cells[MESH]|Amino Acid Sequence[MESH]|Animals[MESH]|Base Sequence[MESH]|DNA, Complementary[MESH]|HT29 Cells[MESH]|Humans[MESH]|Mice[MESH]|Molecular Sequence Data[MESH]|Open Reading Frames[MESH]|RNA, Messenger/genetics/*physiology[MESH]|Tumor Necrosis Factor-alpha/*physiology[MESH] |
