Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Transcriptional control of Borna disease virus (BDV) in persistently BDV-infected cells Mizutani T; Inagaki H; Hayasaka D; Shuto S; Minakawa N; Matsuda A; Kariwa H; Takashima IArch Virol 1999[]; 144 (10): 1937-46Regulation of viral RNA levels in infected cells is considered important in the investigation of viral transcription and replication. Amounts of Borna disease virus (BDV) RNAs were increased in confluent persistently BDV-infected MDCK cells (MDCK/BDV) cells, while maintained at low levels in growing cells. The amount of 1.9-kb RNA without cap formation and polyadenylation at the 5' and 3' ends respectively were remarkably increased (200% per day) in confluent MDCK/BDV cells. Both the full-length genomic and anti-genomic RNAs were increased accompained by 1.9-kb RNA, suggesting the transcription of the 1.9-kb RNA was important for replication of BDV. Ribavirin has an inhibitory effect on replication and transcription of BDV at concentrations from 1 to 10 microgram/ml [Mizutani T et al., Arch Virol (1998)143: 2039-2 044]. BDV transcripts were decreased with ribavirin treatment and increased after its removal which indicated that ribavirin has a reversible inhibitory effect on BDV transcription. Furthermore, BDV transcription was also decreased by two agents, RMNPA and EICAR, which selectively inhibit enzyme activity related to cap formation at the 5' end of mRNA. On the contrary, when the growing MDCK/BDV cells were treated with actinomycin D, transcripts of BDV RNA were increased for 24 h. These agents and culture conditions in this study were found to be useful tools for up-and down-regulation of BDV transcription in persistently BDV-infected cells.|*Gene Expression Regulation, Viral[MESH]|*Transcription, Genetic/drug effects[MESH]|Animals[MESH]|Blotting, Northern[MESH]|Borna disease virus/*genetics/metabolism/*physiology[MESH]|Cells, Cultured[MESH]|Dactinomycin/pharmacology[MESH]|Dogs[MESH]|Down-Regulation[MESH]|Kidney/cytology[MESH]|RNA, Messenger/genetics/metabolism[MESH]|RNA, Viral/genetics/metabolism[MESH]|Ribavirin/pharmacology[MESH]|Up-Regulation[MESH]|Viral Proteins/genetics/metabolism[MESH]|Virus Replication[MESH] |