Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
 
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve
Pubget Overpricing |  
lüll
Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation Mizutani T; Inagaki H; Hayasaka D; Kariwa H; Takashima IJ Vet Med Sci 1999[Jul]; 61 (7): 831-4Transcription of Borna disease virus (BDV) in persistently infected MDCK (MDCK/BDV) cells increased in the fetal bovine serum free media as detected by Northern blot analysis. Especially, the amount of 1.9-kb RNA without cap formation at the 5' end and polyadenylation at the 3' end, increased as compared to other mRNA molecules of BDV. Growth arrest of MDCK/BDV cells observed in the condition of serum starvation might be important for increasing viral transcription. Since N-cadherin is the responsible factor for cell-to-cell contact, MDCK/BDV cells were cultured in calcium free medium which inhibits the interaction of N-cadherin. However, inhibition of cell-to-cell contact by N-cadherin is not effective on up regulation of viral transcription. Our finding in this study indicates that enhancement of BDV transcription by serum starvation is a useful technique for further investigation in understanding of mechanisms of BDV transcription.|*Culture Media, Serum-Free[MESH]|*Gene Expression Regulation, Viral[MESH]|*Transcription, Genetic[MESH]|Animals[MESH]|Borna disease virus/*genetics[MESH]|Cadherins/pharmacology[MESH]|Calcium/physiology[MESH]|Cattle[MESH]|Cell Communication[MESH]|Cell Division[MESH]|Cell Line[MESH]|Genome, Viral[MESH]|Kidney[MESH] |
