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Elucidation of the genome organization of tobacco mosaic virus Zaitlin MPhilos Trans R Soc Lond B Biol Sci 1999[Mar]; 354 (1383): 587-91Proteins unique to tobacco mosaic virus (TMV)-infected plants were detected in the 1970s by electrophoretic analyses of extracts of virus-infected tissues, comparing their proteins to those generated in extracts of uninfected tissues. The genome organization of TMV was deduced principally from studies involving in vitro translation of proteins from the genomic and subgenomic messenger RNAs. The ultimate analysis of the TMV genome came in 1982 when P. Goelet and colleagues sequenced the entire genome. Studies leading to the elucidation of the TMV genome organization are described below.|*Capsid Proteins[MESH]|*Genome, Viral[MESH]|Electrophoresis, Polyacrylamide Gel[MESH]|Nicotiana/virology[MESH]|Plants, Toxic[MESH]|RNA, Messenger/metabolism[MESH]|RNA, Viral/metabolism/*ultrastructure[MESH]|RNA-Dependent RNA Polymerase/metabolism[MESH]|Tobacco Mosaic Virus/*genetics[MESH]|Viral Proteins/genetics/physiology[MESH] |
